Boston University

Optical Characterization and Nanophotonics Laboratory

Home

About

People

Papers

Research

Facilities

Alumni

Resonant Cavity Imaging Biosensor

Members: Anna K. Swan, M. Selim Ünlü

 

Alumni: Sunmin Ahn, Dave Alan Bergstein, Jeremy Richard Colson, Eva Cornell, Jack De Groot, Julia Rentz Dupuis, Abdol Hossein Entekhabi, Jonathan Gershoni, Bennett B. Goldberg, Laura Hoisington, Rostem Irani, Sohini Mahapatra, Elaine Mau, Daniel McHugh, James Needham, Kristen Pamper, Tane Remington, David Phillip Rozenberg, Michael F. Ruane, Christine Woods

 

The Resonant Cavity Imaging Biosensor (RCIB), detects molecules bound to the surface with the potential for tens of thousands of simultaneous parallel observation sites. Current high throughput microarray technology requires that the target molecules be labeled with a fluorescent dye. In the case of protein targets, the direct labeling may alter the conformation and function of the protein while indirect labeling methods via antibodies suffer from cross reactivity and significant background binding. An optical IR beam couples resonantly through a high finesse optical cavity constructed from Bragg mirrors that contains the microarray surface; the wavelength of the IR beam is swept using a tunable IR laser source; and an IR camera monitors cavity transmittance at each pixel, creating a highly parallel signature of transmittance versus wavelength for the microarray surface. Tens of thousands of spots can be evaluated at once. The reflectors feature a SiO2 top surface for enhanced sensitivity as well as chemical compatibility with existing microarray surface chemistry developed for glass slides. The technique additionally relies on the use of commercial telecommunications hardware that has become readily available in recent years. RCIB improves on existing label-free methods by offering dramatically improved throughput necessary to meet the needs of the microarray user community.

Publications

D. A. Bergstein, E. Ozkumur, A. C. Wu, A. Yalcin, J. Needham, R. Irani, J. Gershoni, B. B. Goldberg, C. DeLisi, M. F. Ruane, and M. S. Ünlü, "Resonant Cavity Imaging: A Means Toward High-Throughput Label-Free Protein Detection," IEEE Journal of Selected Topics in Quantum Electronics, Vol. 14, No. 1, January/February 2008

M. S. Ünlü, E. Ozkumur, D. A. Bergstein, A. Yalcin, M. F. Ruane, and B. B. Goldberg, "Applications of Optical Resonance to Biological Sensing and Imaging: II. Resonant Cavity Biosensors," Biophotonics, Biological and Medical Physics, Biomedical Engineering Series, 2008

M. S. Ünlü, E. Ozkumur, J. Needham, D. A. Bergstein, B. B. Goldberg, A. Yalcin, P. S. Spuhler, R. Irani, and C. DeLisi, "Applications of Optical Resonance to Biological Imaging and Label-free Protein Microarrays (invited paper)," EMBC08, Vancouver, Canada, August 20-24, 2008, 2008

D. A. Bergstein, "Resonant Cavity Enhanced Multi-Analyte Sensing," Ph.D. Dissertation, January 2007

D. A. Bergstein, R. Irani, M. F. Ruane, B. B. Goldberg, C. DeLisi, and M. S. Ünlü, "Label-Free Biomolecule Affinity Sensing," 2007 Photonics West Biomedical Optics Symposia, 20-25th January 2007

M. S. Ünlü, and B. B. Goldberg, "Applications of Optical Resonance to Biological Sensing and Imaging (invited)," OSA Frontiers in Optics 2006/Laser Science XXII, Rochester, New York, October 8-12, 2006., 8-12 October 2006

M. S. Ünlü, and B. B. Goldberg, "Applications of Optical Resonance to Biological Sensing and Imaging (invited)," OSA Frontiers in Optics 2006/Laser Science XXII, Rochester, New York, October 8-12, 2006., 2006

D. A. Bergstein, M. F. Ruane, and M. S. Ünlü, "Silicon Substrates with Buried Distributed Bragg Reflectors for Biosensing," International Semiconductor Device Research Symposium 2005, 7-9 December 2005

M. S. Ünlü, "Applications of microresonators: from photodetectors to biological sensing and imaging (Keynote Presentation)," European Optical Society Topical Meeting: Optical Microsystems, 15 September 2005

D. A. Bergstein, M. F. Ruane, B. B. Goldberg, and M. S. Ünlü, "Resonant Cavity Imaging Biosensor," IEEE Lightwave Technologies in Instrumentation & Measurement Conference 2004, 19th & 20th October 2004


© 2007 Trustees of Boston University. All rights reserved.  |  Last modified April 16, 2007 at 12:00 AM EDT