Optical Characterization and Nanophotonics Laboratory

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Resonant Cavity Imaging Biosensor

Members: Sunmin Ahn, Leslie Baggesen, Eva Cornell, George Gaby Daaboul, Jack De Groot, Bennett B. Goldberg, Rostem Irani, Carlos Lopez, Daniel McHugh, Emre Ozkumur, Kristen Pamper, Alexander Reddington, Michael F. Ruane, Karishma Sekhon, Anna K. Swan, M. Selim Ünlü, Rahul Srinivasa Vedula, Xirui Zhang

Alumni: Dave Alan Bergstein, Jeremy Richard Colson, Julia Rentz Dupuis, Abdol Hossein Entekhabi, Jonathan Gershoni, Laura Hoisington, Sohini Mahapatra, Elaine Mau, James Needham, Tane Remington, David Phillip Rozenberg, Christine Woods

The Resonant Cavity Imaging Biosensor (RCIB), detects molecules bound to the surface with the potential for tens of thousands of simultaneous parallel observation sites. Current high throughput microarray technology requires that the target molecules be labeled with a fluorescent dye. In the case of protein targets, the direct labeling may alter the conformation and function of the protein while indirect labeling methods via antibodies suffer from cross reactivity and significant background binding. An optical IR beam couples resonantly through a high finesse optical cavity constructed from Bragg mirrors that contains the microarray surface; the wavelength of the IR beam is swept using a tunable IR laser source; and an IR camera monitors cavity transmittance at each pixel, creating a highly parallel signature of transmittance versus wavelength for the microarray surface. Tens of thousands of spots can be evaluated at once. The reflectors feature a SiO2 top surface for enhanced sensitivity as well as chemical compatibility with existing microarray surface chemistry developed for glass slides. The technique additionally relies on the use of commercial telecommunications hardware that has become readily available in recent years. RCIB improves on existing label-free methods by offering dramatically improved throughput necessary to meet the needs of the microarray user community.

Publications

E. Ozkumur, A. Yalcin, M. Cretich, C. Lopez, D. A. Bergstein, B. B. Goldberg, M. Chiari, and M. S. Ünlü, "Quantification of DNA and protein adsorption by optical phase shift ," Biosensors and Bioelectronics, Vol. 25, September 2009, pp. 167-172

E. Ozkumur, A. Yalcin, M. Cretich, M. S. Ünlü, and M. Chiari, "Label-free, dynamic and quantitative measurement of biomolecular interactions," 23rd International Symposium on MicroScale Bioseparations (MSB) 2009, February 2009

E. Ozkumur, A. Yalcin, F. Damin, B. B. Goldberg, M. Chiari, and M. S. Ünlü, "Label-free and dynamic measurement of biomolecular interactions for high-throughput diagnostics," Photonics West 2009 - BIOS, January 2009

E. Ozkumur, J. Needham, D. A. Bergstein, R. Gonzalez, M. Cabodi, J. Gershoni, B. B. Goldberg, and M. S. Ünlü, "Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications," Proceedings of the National Academy of Science, Vol. 105, 10 June 2008, pp. 7988-7992

D. A. Bergstein, E. Ozkumur, A. C. Wu, A. Yalcin, J. Needham, R. Irani, J. Gershoni, B. B. Goldberg, C. DeLisi, M. F. Ruane, and M. S. Ünlü, "Resonant Cavity Imaging: A Means Toward High-Throughput Label-Free Protein Detection," IEEE Journal of Selected Topics in Quantum Electronics, Vol. 14, No. 1, January/February 2008

E. Ozkumur, J. Needham, D. A. Bergstein, B. B. Goldberg, and M. S. Ünlü, "Label-free real-time microarray imaging using spectral reflectivity information," Photonics West 2008 - BIOS, January 2008

E. Ozkumur, J. Needham, D. A. Bergstein, M. F. Ruane, B. B. Goldberg, and M. S. Ünlü, "Label-free and high-throughput screening of biomolecular interactions," Proceedings of CLEO/QELS 2008, 2008

M. S. Ünlü, E. Ozkumur, D. A. Bergstein, A. Yalcin, M. F. Ruane, and B. B. Goldberg, "Applications of Optical Resonance to Biological Sensing and Imaging: II. Resonant Cavity Biosensors," Biophotonics, Biological and Medical Physics, Biomedical Engineering Series, 2008

M. S. Ünlü, E. Ozkumur, J. Needham, D. A. Bergstein, B. B. Goldberg, A. Yalcin, P. S. Spuhler, R. Irani, and C. DeLisi, "Applications of Optical Resonance to Biological Imaging and Label-free Protein Microarrays (invited paper)," EMBC08, Vancouver, Canada, August 20-24, 2008, 2008

E. Ozkumur, J. Needham, D. A. Bergstein, A. C. Wu, M. F. Ruane, B. B. Goldberg, and M. S. Ünlü, "Label-free microarray imaging using spectral reflectance information," 2007 BMES Annual Fall Meeting, September 2007

D. A. Bergstein, "Resonant Cavity Enhanced Multi-Analyte Sensing," Ph.D. Dissertation, January 2007

D. A. Bergstein, R. Irani, M. F. Ruane, B. B. Goldberg, C. DeLisi, and M. S. Ünlü, "Label-Free Biomolecule Affinity Sensing," 2007 Photonics West Biomedical Optics Symposia, 20-25th January 2007

M. S. Ünlü, and B. B. Goldberg, "Applications of Optical Resonance to Biological Sensing and Imaging (invited)," OSA Frontiers in Optics 2006/Laser Science XXII, Rochester, New York, October 8-12, 2006., 8-12 October 2006

D. A. Bergstein, M. F. Ruane, and M. S. Ünlü, "Silicon Substrates with Buried Distributed Bragg Reflectors for Biosensing," International Semiconductor Device Research Symposium 2005, 7-9 December 2005

M. S. Ünlü, "Applications of microresonators: from photodetectors to biological sensing and imaging (Keynote Presentation)," European Optical Society Topical Meeting: Optical Microsystems, 15 September 2005

D. A. Bergstein, M. F. Ruane, B. B. Goldberg, and M. S. Ünlü, "Resonant Cavity Imaging Biosensor," IEEE Lightwave Technologies in Instrumentation & Measurement Conference 2004, 19th & 20th October 2004